OrangerV1, Main, Exploration, bibRecord, 002E89

Molecular cloning and characterization of a novel gene encoding limonoid UDP-glucosyltransferase in Citrus

Identifieur interne : 002E89 ( Main/Exploration ); précédent : 002E88; suivant : 002E90

Molecular cloning and characterization of a novel gene encoding limonoid UDP-glucosyltransferase in Citrus

Auteurs : Masayuki Kita [Japon] ; Yutaka Hirata [Japon] ; Takaya Moriguchi [Japon] ; Tomoko Endo-Inagaki [Japon] ; Ryoji Matsumoto [Japon] ; Shin Hasegawa [États-Unis] ; Charles G. Suhayda [États-Unis] ; Mitsuo Omura [Japon]

Source :

FEBS Letters [ 0014-5793 ] ; 2000.

RBID : ISTEX:CA17E300D89C01DCEB0F75CCCEA0454770E272E8

English descriptors

KwdEn :
- Amino Acid Sequence, Base Sequence, Blotting, Northern, Blotting, Southern, Citrus (chemistry), Citrus (enzymology), Citrus (metabolism), Cloning, Molecular, Glucosyltransferases (chemistry), Glucosyltransferases (genetics), Glucosyltransferases (isolation & purification), Glucosyltransferases (metabolism), Limonins, Molecular Sequence Data, RNA, Plant (analysis), Sequence Alignment, Sequence Analysis, Protein, Triterpenes (chemistry), Triterpenes (isolation & purification), Triterpenes (metabolism).
MESH :
- chemical , analysis : RNA, Plant.
- chemical , chemistry : Glucosyltransferases, Triterpenes.
- chemistry : Citrus.
- enzymology : Citrus.
- chemical , genetics : Glucosyltransferases.
- chemical , isolation & purification : Glucosyltransferases, Triterpenes.
- metabolism : Citrus, Glucosyltransferases, Triterpenes.
- Amino Acid Sequence, Base Sequence, Blotting, Northern, Blotting, Southern, Cloning, Molecular, Limonins, Molecular Sequence Data, Sequence Alignment, Sequence Analysis, Protein.

Abstract

We isolated a cDNA clone encoding limonoid UDP-glucosyltransferase (limonoid GTase) from the albedo of Satsuma mandarin (Citrus unshiu Marc.) and investigated the contribution to limonoid glucoside accumulation in fruit. The isolated cDNA clone (CitLGT) was 1732 bp in length encoding 511 deduced amino acids with a predicted molecular mass of 57.5 kDa. The products of in vitro translation from an expression vector had the limonoid GTase activity. Southern blot analysis of genomic DNA indicated that CitLGT was present as a single copy gene in the Citrus genome. The amount of transcript corresponding to CitLGT mRNA changed the same way as the fluctuation of limonin glucoside content during fruit development of navel orange (Citrus sinensis Osb.). This indicates that the transcription of CitLGT regulates the conversion of limonoid aglycones to glucosides in citrus fruit.

Url:

https://api.istex.fr/document/CA17E300D89C01DCEB0F75CCCEA0454770E272E8/fulltext/pdf

DOI: 10.1016/S0014-5793(00)01275-8

Affiliations:

Le document en format XML

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<author><name sortKey="Moriguchi, Takaya" sort="Moriguchi, Takaya" uniqKey="Moriguchi T" first="Takaya" last="Moriguchi">Takaya Moriguchi</name>
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<author><name sortKey="Endo Inagaki, Tomoko" sort="Endo Inagaki, Tomoko" uniqKey="Endo Inagaki T" first="Tomoko" last="Endo-Inagaki">Tomoko Endo-Inagaki</name>
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<author><name sortKey="Hasegawa, Shin" sort="Hasegawa, Shin" uniqKey="Hasegawa S" first="Shin" last="Hasegawa">Shin Hasegawa</name>
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<author><name sortKey="Suhayda, Charles G" sort="Suhayda, Charles G" uniqKey="Suhayda C" first="Charles G." last="Suhayda">Charles G. Suhayda</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Base Sequence</term>
<term>Blotting, Northern</term>
<term>Blotting, Southern</term>
<term>Citrus (chemistry)</term>
<term>Citrus (enzymology)</term>
<term>Citrus (metabolism)</term>
<term>Cloning, Molecular</term>
<term>Glucosyltransferases (chemistry)</term>
<term>Glucosyltransferases (genetics)</term>
<term>Glucosyltransferases (isolation & purification)</term>
<term>Glucosyltransferases (metabolism)</term>
<term>Limonins</term>
<term>Molecular Sequence Data</term>
<term>RNA, Plant (analysis)</term>
<term>Sequence Alignment</term>
<term>Sequence Analysis, Protein</term>
<term>Triterpenes (chemistry)</term>
<term>Triterpenes (isolation & purification)</term>
<term>Triterpenes (metabolism)</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Glucosyltransferases</term>
<term>Triterpenes</term>
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<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glucosyltransferases</term>
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<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Glucosyltransferases</term>
<term>Triterpenes</term>
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<term>Glucosyltransferases</term>
<term>Triterpenes</term>
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<term>Base Sequence</term>
<term>Blotting, Northern</term>
<term>Blotting, Southern</term>
<term>Cloning, Molecular</term>
<term>Limonins</term>
<term>Molecular Sequence Data</term>
<term>Sequence Alignment</term>
<term>Sequence Analysis, Protein</term>
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<front><div type="abstract" xml:lang="en">We isolated a cDNA clone encoding limonoid UDP-glucosyltransferase (limonoid GTase) from the albedo of Satsuma mandarin (Citrus unshiu Marc.) and investigated the contribution to limonoid glucoside accumulation in fruit. The isolated cDNA clone (CitLGT) was 1732 bp in length encoding 511 deduced amino acids with a predicted molecular mass of 57.5 kDa. The products of in vitro translation from an expression vector had the limonoid GTase activity. Southern blot analysis of genomic DNA indicated that CitLGT was present as a single copy gene in the Citrus genome. The amount of transcript corresponding to CitLGT mRNA changed the same way as the fluctuation of limonin glucoside content during fruit development of navel orange (Citrus sinensis Osb.). This indicates that the transcription of CitLGT regulates the conversion of limonoid aglycones to glucosides in citrus fruit.</div>
</front>
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<tree><country name="Japon"><noRegion><name sortKey="Kita, Masayuki" sort="Kita, Masayuki" uniqKey="Kita M" first="Masayuki" last="Kita">Masayuki Kita</name>
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<name sortKey="Endo Inagaki, Tomoko" sort="Endo Inagaki, Tomoko" uniqKey="Endo Inagaki T" first="Tomoko" last="Endo-Inagaki">Tomoko Endo-Inagaki</name>
<name sortKey="Hirata, Yutaka" sort="Hirata, Yutaka" uniqKey="Hirata Y" first="Yutaka" last="Hirata">Yutaka Hirata</name>
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<name sortKey="Moriguchi, Takaya" sort="Moriguchi, Takaya" uniqKey="Moriguchi T" first="Takaya" last="Moriguchi">Takaya Moriguchi</name>
<name sortKey="Omura, Mitsuo" sort="Omura, Mitsuo" uniqKey="Omura M" first="Mitsuo" last="Omura">Mitsuo Omura</name>
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<country name="États-Unis"><region name="Californie"><name sortKey="Hasegawa, Shin" sort="Hasegawa, Shin" uniqKey="Hasegawa S" first="Shin" last="Hasegawa">Shin Hasegawa</name>
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<name sortKey="Suhayda, Charles G" sort="Suhayda, Charles G" uniqKey="Suhayda C" first="Charles G." last="Suhayda">Charles G. Suhayda</name>
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Molecular cloning and characterization of a novel gene encoding limonoid UDP-glucosyltransferase in Citrus

Molecular cloning and characterization of a novel gene encoding limonoid UDP-glucosyltransferase in Citrus

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Abstract

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